Gel purification – Freeze & Squeeze method

Very fast gel purification by freeze & squeeze

Gel purification by the Freeze & Squeeze method is probably the fastest way to get DNA out of a gel. This gel purification method is also the least labor intensive method. The video below shows you how to make filters for Freeze & Squeeze gel extraction and how to perform the extraction

Advantages of the Freeze & Squeeze gel purification method

  1. Very fast (just over 8 minutes)
  2. Minimally labor intensive
  3. Very cheap (about 4 cents)

Download a print protocol for the Freeze and Squeeze Gel Extraction here.

14 thoughts on “Gel purification – Freeze & Squeeze method”

    1. Sure. However, I would not recommend it since it is extra unnecessary work that will also result in less yield.

    1. Yes, it works for high molecular weight DNA. I have purified 12 Kb bands with this method. However, the yield does go down as molecular weight goes up, so you may need to scale up a little if working with large DNA fragments.

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  3. Hi rahul.
    First of all, thanks for sharing this protocol. Its very easy, fast, cheap and it will be very useful for me.
    By the other hand, I was looking your website and I found it very interesting; you describe almost protocols necesaries in molecular cloning, but unfortunately I didn’t find a protocol in your website about setting a DNA ligation.
    When you have a little chance,
    Could you kindly explain me how to set one? It will helps me a lot.
    Thanks for your atention.
    Best regards.
    Adrián Saldaña

  4. Is it possible to freeze the tubes for a longer period of time in a regular freezer or does it has to be -80 degrees?

    1. 1.2% or less. I like to use 1.0% gels for most purposes. However, most agarose percentages can work but high percentages typically result in less yield.

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