Very fast gel purification by freeze & squeeze
Gel purification by the Freeze & Squeeze method is probably the fastest way to get DNA out of a gel. This gel purification method is also the least labor intensive method. The video below shows you how to make filters for Freeze & Squeeze gel extraction and how to perform the extraction
Advantages of the Freeze & Squeeze gel purification method
- Very fast (just over 8 minutes)
- Minimally labor intensive
- Very cheap (about 4 cents)
Download a print protocol for the Freeze and Squeeze Gel Extraction here.
Could one then pipet the DNA onto a Quick Gel Extraction Column?
Sure. However, I would not recommend it since it is extra unnecessary work that will also result in less yield.
HI, Would this work for purification of high molecular weight DNA ?
Yes, it works for high molecular weight DNA. I have purified 12 Kb bands with this method. However, the yield does go down as molecular weight goes up, so you may need to scale up a little if working with large DNA fragments.
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Can this be used to extract and purify heterodimers and small molecule aptamer complexes?
I am also curious. I may try shortly…
Hi rahul.
First of all, thanks for sharing this protocol. Its very easy, fast, cheap and it will be very useful for me.
By the other hand, I was looking your website and I found it very interesting; you describe almost protocols necesaries in molecular cloning, but unfortunately I didn’t find a protocol in your website about setting a DNA ligation.
When you have a little chance,
Could you kindly explain me how to set one? It will helps me a lot.
Thanks for your atention.
Best regards.
Adrián Saldaña
I will try to post this when a have time to make the video.
Is it possible to freeze the tubes for a longer period of time in a regular freezer or does it has to be -80 degrees?
Yes, you can freeze longer in a -20 degree freezer and that will also work. Just make sure the gel is frozen.
What percent agarose do you recommend for this method? Is <1.5% ideal or can it work for 3-4.5%?
1.2% or less. I like to use 1.0% gels for most purposes. However, most agarose percentages can work but high percentages typically result in less yield.