Very fast gel purification by freeze & squeeze
Gel purification by the Freeze & Squeeze method is probably the fastest way to get DNA out of a gel. This gel purification method is also the least labor intensive method. The video below shows you how to make filters for Freeze & Squeeze gel extraction and how to perform the extraction
Advantages of the Freeze & Squeeze gel purification method
- Very fast (just over 8 minutes)
- Minimally labor intensive
- Very cheap (about 4 cents)
Download a print protocol for the Freeze and Squeeze Gel Extraction here.
Could one then pipet the DNA onto a Quick Gel Extraction Column?
Sure. However, I would not recommend it since it is extra unnecessary work that will also result in less yield.
HI, Would this work for purification of high molecular weight DNA ?
Yes, it works for high molecular weight DNA. I have purified 12 Kb bands with this method. However, the yield does go down as molecular weight goes up, so you may need to scale up a little if working with large DNA fragments.
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Can this be used to extract and purify heterodimers and small molecule aptamer complexes?
I am also curious. I may try shortly…
Hi rahul.
First of all, thanks for sharing this protocol. Its very easy, fast, cheap and it will be very useful for me.
By the other hand, I was looking your website and I found it very interesting; you describe almost protocols necesaries in molecular cloning, but unfortunately I didn’t find a protocol in your website about setting a DNA ligation.
When you have a little chance,
Could you kindly explain me how to set one? It will helps me a lot.
Thanks for your atention.
Best regards.
Adrián Saldaña
I will try to post this when a have time to make the video.
Is it possible to freeze the tubes for a longer period of time in a regular freezer or does it has to be -80 degrees?
Yes, you can freeze longer in a -20 degree freezer and that will also work. Just make sure the gel is frozen.
What percent agarose do you recommend for this method? Is <1.5% ideal or can it work for 3-4.5%?
1.2% or less. I like to use 1.0% gels for most purposes. However, most agarose percentages can work but high percentages typically result in less yield.
Hello – very cool resourcefulness. Can this approach also work on RNA gel extractions?
Yes but you will have to take the considerations for RNA stability.
Any thoughts about the percentage of recovery from the gel…I am curious to know?
IT varies by size. Recovery of small DNA pieces (1 kb and under) could easily be above 90%. However, for large DNA recovery can be much less.