Transient Transformation of Wild Tobacco by Agroinfiltration
Transiently transforming Nicotiana benthamiana (wild tobacco) by agroinfiltration is an easy way to quickly express genes for protein localization or gene function studies. This video shows you how to perform tobacco agroinfiltration.
Download a written protocol for Tobacco Agroinfiltration here.
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Thank you for the nice words! We are very happy that it helps you.
Simple and Easy protocol… Thank you for sharing…
Your are welcome.
how you calculate the amount of bacteia with gene of interest and how much of p19 and how much infiltration media to disolve to which OD600: 0,5 or 1…?
Please check the protocol carefully. It has this information. You use a spec. to estimate bacterial concentration.