Inoculating liquid cultures with a single bacterial colony.
Author: rahulpatharkar
Determining MW of proteins on SDS-PAGE
This video shows you how to determine the molecular weight (MW) of a protein that was run on an SDS-PAGE gel. Note that the exact same technique can be used for determining the size of DNA fragments on agarose gels.
Setting up an analytical restriction digest
Setting up an analytical restriction digest.
Download and Excel file to automatically calculate Master-mix volumes here. You could run your digests on slide agarose gels or standard agarose gels to resolve the DNA species. Slide agarose gels are faster and are my desired gel system for all DNA and RNA applications.
E. coli transformation and plating
Download a printable E. coli transformation protocol here.
I have found that the Inoue, H., Nojima, H., & Okayama, H. (1990). Gene, 96(1), 23-28. method gives the highest efficiency competent cells and is very easy to do. You can get a protocol for the Inoue method here.
Pouring LB agar plates
Making LB agar plates for growing bacteria.
Download an Excel sheet calculator to help make your LB agar media.
Plant genomic DNA extraction
The CTAB method yields high quality plant genomic DNA that can be used for virtually any application including Illumina sequencing. The method is fast, cheap, and scalable. For genotyping, less than 1 mm x 1 mm pieces of plant material can be use.
Download a printable CTAB plant genomic DNA extraction protocol here.