e. coli - Rahul Patharkar

Inoculating plasmid minipreps

Inoculating liquid cultures with a single bacterial colony.

E. coli transformation and plating

Transforming E. coli with plasmids and plating the transformation.

Download a printable E. coli transformation protocol here.

I have found that the Inoue, H., Nojima, H., & Okayama, H. (1990). Gene, 96(1), 23-28. method gives the highest efficiency competent cells and is very easy to do. You can get a protocol for the Inoue method here.