I have found that the Inoue, H., Nojima, H., & Okayama, H. (1990). Gene, 96(1), 23-28. method gives the highest efficiency competent cells and is very easy to do. You can get a protocol for the Inoue method here.
Making LB agar plates for growing bacteria.
Download an Excel sheet calculator to help make your LB agar media.
The CTAB method yields high quality plant genomic DNA that can be used for virtually any application including Illumina sequencing. The method is fast, cheap, and scalable. For genotyping, less than 1 mm x 1 mm pieces of plant material can be use.
Download a printable CTAB plant genomic DNA extraction protocol here.
